Partners Media Releases Contact Us


Since its establishment, CUBEBIO has committed itself to research and development of cancer biomarkers and products for early detection of cancer.

KIT tool

Cancer Diagnostic Kit


Colorimetric Analysis based on an enzymatic reaction

How it works

The appearance and growth of cancer cells within the body cause various substances
to be metabolized and excreted in urine. During the developing stage of cancer, amino acid,
glucose, nucleic acid, and protein among other elements are metabolized in an unusually
large quantity and excreted with an abnormality in urine concentration. We can check
the possibility of the existence of cancer by measuring the difference in urine concentration.

How to use

Utilizing an analysis method developed by CUBEBIO (Quantitative test using enzymes in urine), we mix 10μl of urine and 90μl of compounds made with enzymatic reactions.
After blocking out light for 5 minutes at room temperature (25 °C), we use ELISA reader to measure the level of absorbance at 450 nm.

Fill a 96 well microplate with urine

Mix urine and CUBEBIO's compounds made
with enzymatic reactions

After blocking out light for 5 minutes at room
temperature (25 °C), use ELISA reader and
measure the level of absorbance

Interpret the test result(Checking the concentration
of a cancer biomarker by using the level of absorbance)

Test result Quantifying P-marker through colorimetric analysis
by urinary enzymatic reaction, it is possible to distinguish
between cancer and non-cancer on the basis of high
specificity and sensitivity.