Cancer Diagnostic Kit
MethodColorimetric Analysis based on an enzymatic reaction
How it worksThe appearance and growth of cancer cells within the body cause various substances
to be metabolized and excreted in urine. During the developing stage of cancer, amino acid,
glucose, nucleic acid, and protein among other elements are metabolized in an unusually
large quantity and excreted with an abnormality in urine concentration. We can check
the possibility of the existence of cancer by measuring the difference in urine concentration.
How to useUtilizing an analysis method developed by CUBEBIO (Quantitative test using enzymes in urine), we mix 10μl of urine and 90μl of compounds made with enzymatic reactions.
After blocking out light for 5 minutes at room temperature (25 °C), we use ELISA reader to measure the level of absorbance at 450 nm.
Fill a 96 well microplate with urine
Mix urine and CUBEBIO's compounds made
with enzymatic reactions
After blocking out light for 5 minutes at room
temperature (25 °C), use ELISA reader and
measure the level of absorbance
Interpret the test result(Checking the concentration
of a cancer biomarker by using the level of absorbance)
by urinary enzymatic reaction, it is possible to distinguish
between cancer and non-cancer on the basis of high
specificity and sensitivity.